PNAClamp™ Mutation Detection Kit BCR-ABL
Ref. PNAC-7001CML (Chronic myelogenous leukemia) patients who has consistently treated with Imatinib (Gleevec), have acquired point mutations in tyrosine kinase domain of BCR-ABL fusion gene. Especially, T315I mutation is known as a mutation associated with Imatinibresistance and observed with high frequency.
About the Product
CML (Chronic myelogenous leukemia) patients who has consistently treated with Imatinib (Gleevec), have acquired point mutations in tyrosine kinase domain of BCR-ABL fusion gene. Especially, T315I mutation is known as a mutation associated with Imatinibresistance and observed with high frequency.
It is important to monitor patients treated with Imatinibby BCR-ABLfusion gene mutation test to prevent therapy failure due to drug resistance by mutation.
BCR-ABL T315I can be detected by PNAClamp™ BCR-ABL Mutation Detection Kit.
Technical Characteristics
| Mutations - Exon 6 | T315I (944C) |
|---|---|
| Detection limit | 1% LOD with 10 ng wild/mutant type mixed DNA |
| Running time | within 3 hours |
| Procedure | sample preparationExtraction of Total DNAAmplification of target DNA by Real-Time PCRAnalysis of Result |
Key Features
- High sensitivity and specificity even with small amount of DNA (1% LOD with 10 ng wild/mutant type mixed DNA)
- Real-Time PCR based ready-to-use kit
- Short running time (within 3 hours)
Technical Information
No technical information available for this product.
Compatibility
Compatible sample type: Whole Blood
Compatible Real-Time PCR machine:
- CFX96 (Bio-Rad)
- LightCycler 480II (Roche)
- ABI7500 (Thermo Fisher Scientific)
- ABI7900HT (Thermo Fisher Scientific)
- StepOnePlus (Thermo Fisher Scientific)
- QuantStudio 5 (Thermo Fisher Scientific)
- Rotor-Gene Q (Qiagen)
Packaging
25 tests