PNAClamp™ Mutation Detection Kit JAK2
Ref. PNAC-6001JAK2 V617F mutation is found in patients with blood related disease such as Polycythemia Vera, Essential Thrombocythemia and Myelofibrosis. Ruxolitinib is effective as a JAK Inhibitor, so that it is a prognostic factor to determine drug response.
About the Product
JAK2 V617F mutation is found in patients with blood related disease such as Polycythemia Vera, Essential Thrombocythemia and Myelofibrosis. Ruxolitinib is effective as a JAK Inhibitor, so that it is a prognostic factor to determine drug response.
JAK2 V617F mutation detection makes easy to classify patients with MPD (Myeloproliferative Disease) as three levels of diagnostic certainty (possible, probable and definite) and to determine which signal transduction therapy is suitable for each patients. In addition, testing for JAK2 V617F mutation has been recommended as clinical tests by WHO(World Health Organization).
JAK2 V617F mutation can be detected by PNAClamp™ JAK2 Mutation Detection Kit.
Technical Characteristics
| Mutations - Exon 14 | V617F (1849G>T) |
|---|---|
| Detection limit | 1% LOD with 10 ng wild/mutant type mixed DNA |
| Running time | within 3 hours |
| Procedure | sample preparationExtraction of Total DNAAmplification of target DNA by Real-Time PCRAnalysis of Result |
Key Features
- High sensitivity and specificity even with small amount of DNA (1% LOD with 10 ng wild/mutant type mixed DNA)
- Real-Time PCR based ready-to-use kit
- Short running time (within 3 hours)
Technical Information
No technical information available for this product.
Compatibility
Compatible sample type: Blood
Compatible Real-Time PCR machine:
- CFX96 (Bio-Rad)
- LightCycler 480II (Roche)
- ABI7500 (Thermo Fisher Scientific)
- ABI7900HT (Thermo Fisher Scientific)
- Rotor-Gene Q (Qiagen)
Packaging
25 tests