PNAClamp™ Mutation Detection Kit IDH2
Ref. PNAC-5101IDH2 is frequently mutated in glioma, acute myeloid leukemia, and myelodysplastic syndromes. Mutations generally involve point mutations at the R140 and R172 residues of the protein.
About the Product
IDH2 is frequently mutated in glioma, acute myeloid leukemia, and myelodysplastic syndromes. Mutations generally involve point mutations at the R140 and R172 residues of the protein. Mutations in IDH1 and IDH2 result in deleterious “gain of function”: instead of converting isocitrate to α-ketoglutarate, mutated IDH1 or IDH2 converts isocitrate to 2-hydroxyglutarate. 2-hydroxyglutarate inhibits other proteins involved in epigenetic regulation.
Total 11 IDH2 mutations can be detected.
Technical Characteristics
| Mutations - Codon 140 | 7 |
|---|---|
| Mutations - Codon 172 | 4 |
| Total mutations | 11 |
| Detection limit | 1% LOD with 10 ng wild/mutant type mixed DNA |
| Running time | within 3 hours |
| Procedure | sample preparationExtraction of Total DNAAmplification of target DNA by Real-Time PCRAnalysis of Result |
Key Features
- High sensitivity and specificity even with small amount of DNA (1% LOD with 10 ng wild/mutant type mixed DNA)
- Real-Time PCR based ready-to-use kit
- Short running time (within 3 hours)
Technical Information
No technical information available for this product.
Compatibility
Compatible sample type:
- FFPE tissue
- Biopsy tissue
Compatible Real-Time PCR machine:
- CFX96 (Bio-Rad)
- LightCycler 480II (Roche)
- ABI7500 (Thermo Fisher Scientific)
- ABI7900HT (Thermo Fisher Scientific)
- StepOnePlus (Thermo Fisher Scientific)
- QuantStudio 5 (Thermo Fisher Scientific)
- Rotor-Gene Q (Qiagen)
Packaging
25 tests