PNAClamp™ Mutation Detection Kit BRAF
Ref. PNAC-2001BRAF mutation is found in several cancers including thyroid cancer, malignant melanoma, ovarian cancer, colorectal cancer. In case of thyroid cancer, there are over 40 different types of BRAF mutation. Of these mutations, the V600E mutation on exon 15, i.e.
About the Product
BRAF mutation is found in several cancers including thyroid cancer, malignant melanoma, ovarian cancer, colorectal cancer. In case of thyroid cancer, there are over 40 different types of BRAF mutation. Of these mutations, the V600E mutation on exon 15, i.e. change of nucleotide from T to A in 1799th base that results in change from valine to glutamate in amino acids, is most important with over 90% of frequency. Especially, BRAF V600E mutation is detected with ca. 45% of frequency in papillary thyroid carcinoma and is considered a prognostic maker for thyroid cancer.
Total 5 BRAF mutations can be detected.
Technical Characteristics
| Mutations | p.V600E, p.V600D, p.V600K, p.V600R, p.V600A |
|---|---|
| Total mutations | 5 |
| Detection limit | 1% LOD with 10 ng wild/mutant type mixed DNA |
| Running time | within 3 hours |
| Procedure | DNA ExtractionPCR SetupReal-time PCRResult Analysis |
Key Features
- High sensitivity and specificity even with small amount of DNA (1% LOD with 10 ng wild/mutant type mixed DNA)
- Real-Time PCR based ready-to-use kit
- Short running time (within 3 hours)
Technical Information
No technical information available for this product.
Compatibility
Compatible sample type:
- FFPE tissue
- Fresh tissue
Compatible Real-Time PCR machine:
- CFX96 (Bio-Rad)
- LightCycler 480II (Roche)
- ABI7500 (Thermo Fisher Scientific)
- Rotor-Gene Q (Qiagen)
Packaging
50 tests