Tissue biopsy
PNAClamp™ Mutation Detection Kit NRAS
Ref. PNAC-1101NRAS mutation is found in several cancers such as melanoma (13~25%), colorectal cancer (1~6%), lung cancer (1%), thyroid cancer (7%) and hepatocellular carcinoma (10%).
Tests per card
—
Packaging
25 tests
Contact us for product availability and technical details.
About the Product
NRAS mutation is found in several cancers such as melanoma (13~25%), colorectal cancer (1~6%), lung cancer (1%), thyroid cancer (7%) and hepatocellular carcinoma (10%). It is know that the drug response against colorectal cancer medicine such as Erbitux and Cetuximab decreased and the prognosis of the metastatic colorectal cancer patient is bad if the patient has NRAS mutation.
Total 43 NRAS mutations can be detected.
Technical Characteristics
| Mutations - Codon 12 | 9 |
|---|---|
| Mutations - Codon 13 | 10 |
| Mutations - Codon 59 | 3 |
| Mutations - Codon 61 | 12 |
| Mutations - Codon 117 | 4 |
| Mutations - Codon 146 | 5 |
| Total mutations | 43 |
| Detection limit | 2% LOD with 10 ng wild/mutant type mixed DNA |
| Running time | within 3 hours |
| Procedure | sample preparationExtraction of Total DNAAmplification of target DNA by Real-Time PCRAnalysis of Result |
Key Features
- High sensitivity and specificity even with small amount of DNA (2% LOD with 10 ng wild/mutant type mixed DNA)
- Real-Time PCR based ready-to-use kit
- Short running time (within 3 hours)
Technical Information
No technical information available for this product.
Compatibility
Compatible sample type: FFPE tissue
Compatible Real-Time PCR machine:
- CFX96 (Bio-Rad)
- LightCycler 480II (Roche)
- ABI7500 (Thermo Fisher Scientific)
- ABI7900HT (Thermo Fisher Scientific)
- StepOnePlus (Thermo Fisher Scientific)
- Rotor-Gene Q (Qiagen)
Packaging
25 tests