PANAMutyper™ R EGFR
Ref. PNAR-3001Lung cancer is a metastatic cancer which the cancer cells are developed in the bronchial tubes or alveoli and grow along the blood vessel or lymphatic vessel.
About the Product
Lung cancer is a metastatic cancer which the cancer cells are developed in the bronchial tubes or alveoli and grow along the blood vessel or lymphatic vessel. There are two types of lung cancer based on the sizes and shape of the cancer cell; non-small cell lung cancer (NSCLC) takes 75% and small cell lung cancer takes 25%. Usually, it is hard to find lung cancer in the early stage and is diagnosed as lung cancer when it is already progressed. So the prognosis tends to be bad. It is known that the patients with EGFR mutation show very high drug responses against EGFR tyrosine kinase inhibitor (TKI) such as Gefitinib (Iressa, AstraZenca) and Erlotinib (Tarceba, Roche). We expect that genotyping EGFR genes of the lung cancer patients enables predicting drug response before treatment and this will lead effective lung cancer treatment.
Total 47 EGFR Mutations can be detected and discriminated.
Technical Characteristics
| Exon 18 - G719X | 3 |
|---|---|
| Exon 19 - E19del | 29 |
| Exon 20 - T790M | 1 |
| Exon 20 - S768I | 1 |
| Exon 20 - E20ins | 10 |
| Exon 21 - L858R | 2 |
| Exon 21 - L861Q | 1 |
| Total mutations | 47 |
| Detection limit | 0.1% LOD with 2 ml plasma |
| Running time | within 3 hours |
| Procedure | sample preparationExtraction of Total DNAreal time PCRAnalysis of Result |
Key Features
- Multiplex genotyping with a sample
- High sensitivity and specificity even with small amount of ctDNA (0.1% LOD with 2 ml plasma)
- Short running time (within 3 hours)
Technical Information
No technical information available for this product.
Compatibility
Compatible sample type:
- FFPE tissue
- Plasma
Compatible Real-Time PCR machine:
- CFX96 (Bio-Rad)
- QuantStudio5 (Thermo Fisher Scientific)
Packaging
24 tests